40X-1000X Trinocular Inverted Fluorescence Microscope with Phase-contrast + 3MP SCMOS Camera

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$1,817.70

40X-1000X Trinocular Inverted Fluorescence Microscope with Phase-contrast + 3MP SCMOS Camera

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Description

The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design.

The microscope uses an infinity-corrected optical system with an expanded magnificatin range of 40X to 1000X. The four plan-fluor objectives lenses provide high-resolution, color-corrected images with edge-to-edge sharpness. The 10X and 20X lenses can be used in conjunction with the provided phase-annulus plate for phase-contrast.

For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

The inverted format provides a platform for a wide range of vessels, including slides, culture dishes and flasks, and Terasaki plates. A camera can be easily mounted to the dedicated photo port for a live video feed and digital imaging. A wide range of cameras is available, including CCD and back-illuminated CMOS models designed specifically for low-light applications.

The 3MP color sCMOS camera is designed for low-light applications. It captures still images and video, and allows you to view a live stream on your PC. The included Windows software offers image-development and measurement tools. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images.

Fluorescence

Filters

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Filter Set Excitation Filter Dichromatic Mirror Emission Filter Common Stains
B 450-490nm 520nm Acridine Orange (DNA), Acridine Yellow, Alexa Fluor (430, 488, 500, 514),Cy2, DiO, FITC, Lucifer Yellow
G 510-560nm 590nm Acridine Red, Alexa Fluor 532, NeuroTrace 530/615, Rhodamine B
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Specifications

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Optical System Infinity-corrected
Head Trinocular, 45° incline
Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm
Ocular Diameter 30mm
Eyepieces 10X FN22mm plan wide-field, 25X wide-field
Photo-port 23mm tube, height-adjustable
Objective Turret quadruple
Focusing System

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Focus Adjustment Coaxial Coarse/Fine, adjustable tension
Fine Division 0.002mm
Coarse Stroke
Fine Stroke
Stage fixed-height, mechanical X-Y translation
Stage Dimensions 172mm x 242mm
Stage X-Y Travel Range 75mm x 40mm
Specimen Holders Terasaki plate, Petri dish
Transmitted Illumination 12V 30W halogenvariable intensitycenterable lamp
Condenser NA 0.3, iris diaphragm, filter slot
Condenser Working Distance
Phase Contrast Annuli 10X and 20X
Power 110VAC 60Hz

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X Plan fluor 0.15 28mm 1.5mm
10X Plan fluor, phase-contrast 0.30 3mm 1.5mm
20X Plan fluor, phase-contrast 0.50 4.27mm 1.5mm
40X Plan fluor 0.60 2.23mm 1.5mm

Magnification Chart

Eyepiece Objective
4X 10X 20X 40X
10X 40X 100X 200X 400X
25X 100X 250X 500X 1000X

Camera Specifications

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Sensor Type sCMOS (color)
Sensor Optical Format 1/2.8″
Active Pixels 3.1M (2048 x 1536)
Pixel Size 2.5µm x 2.5µm
Active Sensor Area 5.12mm x 3.84mm
Shutter electronic rolling shutter
Sensitivity
Spectral Response
Capture Resolution and Maximum Framerate
Connectivity USB 2.0
Lens-mount C-mount
Power 5V over USB

Software

OS Requirements Windows (32/64 bit) XP/Vista/7/8/10
Features
Windows Version
Live video preview
Captures still images and video
Exposure and white-balance controls
Image processing
Measurement tools, including lines, arcs, polygons

Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair of 10X Eyepieces
  • One pair of 25X Eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB cable
  • One software CD
  • Additional information

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    40X-1000X Trinocular Inverted Fluorescence Microscope with Phase-contrast + 3MP SCMOS Camera

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